PP-12 ARTIFICIAL COLLAPSE OF HUMAN BLASTOCYSTS, BEFORE VITRIFICATION, IMPROVES SURVIVAL AND PREGNANCY RATES
نویسندگان
چکیده
منابع مشابه
Cryopreserved human blastocysts after vitrification result in excellent implantation and clinical pregnancy rates.
We conducted a retrospective review of all frozen ETs from May 2002 to October 2007 using our new vitrification technique in order to determine its efficacy. We found high pregnancy (70.5%; 79/112) and implantation (40.6%; 110/271) rates with the use of this new method.
متن کاملEffects of blastocyst artificial collapse prior to vitrification on hatching and survival rates and the expression of klf4 gene in mouse embryos
Although the rate of blastocysts implantation of embryos is higher than previous stages but their survival rate is lower than them, which could be attributed to the completely filled blastocoel cavity with liquid and increased possibility of the formation of ice crystals. This liquid could prevent the penetration of cryoprotecting materials into the embryos. In this study, we reduced the volume...
متن کاملSerum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification.
The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2+5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF)+5% FCS, and SOF+20 gL(-1) bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of ...
متن کاملPregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele.
BACKGROUND The purpose of this study was to examine the effects on blastocyst survival and subsequent pregnancy rate of 'artificial shrinkage' (i.e. induced collapse of the blastocoele) before vitrification of human blastocysts. METHODS After embryo transfer in IVF cycles, surplus embryos that developed to the expanded blastocyst stage were cryopreserved. Before vitrification on electron micr...
متن کاملLong Cut Straw Provides Stable the Rates of Survival, Pregnancy and Live Birth for Vitrification of Human Blasotcysts
Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A to...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2012
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(12)60144-8